Offensive Positions
| POS | Starter | Second | Third+ |
|---|---|---|---|
| Quarterback | M. StaffordMatthew Stafford | J. GaroppoloJimmy Garoppolo | |
| Running Back | K. WilliamsKyren Williams | R. RiversRonnie Rivers | |
| Wide Receiver | P. NacuaPuka Nacua | C. KuppCooper Kupp | |
| Tight End | T. HigbeeTyler Higbee | C. ParkinsonColby Parkinson | |
| Left Tackle | A. JacksonAlaric Jackson | J. JacksonJonah Jackson | |
| Center | B. LimmerBeaux Limmer | J. JacksonJonah Jackson | |
| Right Tackle | R. HavensteinRob Havenstein | J. NoteboomJoe Noteboom |
Defensive Positions
| POS | Starter | Second | Third+ |
|---|---|---|---|
| Left Defensive End | B. FiskeBraden Fiske | T. DavisTyler Davis | — |
| Nose Tackle | B. Brown IIIBobby Brown III | N. GallimoreNeville Gallimore | — |
| Right Defensive End | K. TurnerKobie Turner | D. JohnsonDesjuan Johnson | |
| Left Inside Linebacker | C. RozeboomChristian Rozeboom | J. HummelJake Hummel | — |
| Right Inside Linebacker | O. SpeightsOmar Speights | J. McColloughJaylen McCollough | |
| Strongside Linebacker | J. VerseJared Verse | M. HoechtMike Hoecht | — |
| Weakside Linebacker | B. YoungByron Young | B. JacksonBrennan Jackson | |
| Right Cornerback | A. WitherspoonAhkello Witherspoon | D. DurantDecobie Durant | |
| Left Cornerback | D. WilliamsDarious Williams | J. WallaceJosh Wallace | |
| Slot Cornerback | Q. LakeQuentin Lake | — | — |
| Strong Safety | K. CurlKamren Curl | J. McColloughJaylen McCollough | — |
| Free Safety | K. KinchensKamren Kinchens | J. JohnsonJohn Johnson | — |
Special Teams Positions
| POS | Starter | Second | Third+ |
|---|---|---|---|
| Punter | E. EvansEthan Evans | — | — |
| Kicker | J. KartyJoshua Karty | — | — |
| Long Snapper | A. WardAlex Ward | — | — |
| Holder | E. EvansEthan Evans | — | — |
| Punt Returner | X. SmithXavier Smith | K. WilliamsKyren Williams | — |
| Kick Returner | J. WhittingtonJordan Whittington | X. SmithXavier Smith |